This cell line is able, using the test called 'cell transformation assay', to identify the carcinogenic potential of chemicals in vitro.
The BALB/3T3 is one of several cell lines developed by S.A. Aaronson and G.T. Todaro in 1968 from BALB/c mouse embryos.
BALB/c is an albino, laboratory-bred strain of the House Mouse originated in New York in 1920, from which a number of common substrains are derived.
The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3 x 105 cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 x 105 cells per 20-cm² dish (the second "3") continuously. The spontaneously immortalized cells with stable growth rate were established after 20-30 generations in culture, and then named '3T3' cells.
- Balb/3T3 mouse fibroblasts