European Commission > JRC > IHCP > Our Activities > Nanotechnology > JRC scientists contribute to the synthesis of radiolabelled gold nanoparticle aerosols for rodent inhalation and translocation studies
Personal tools

JRC scientists contribute to the synthesis of radiolabelled gold nanoparticle aerosols for rodent inhalation and translocation studies

— filed under: ,

The paper deals with the production of radiolabelled gold nanoparticle aerosols by spark ignition at the Helmholtz Centre Munich using gold electrodes activated at the JRC’s Cyclotron Facility in Ispra, their characterisation in terms of chemical composition, crystalline structure, morphology, specific surface area and radioactivity, and their interaction with lung tissues and lung cells after 1 h inhalation by mice

The intensive use of nanoparticles (NP) in many different applications necessitates studies on their risk assessment as there are still open questions on their safe handling and utilization. For reliable risk assessment, the interaction of NP with biological systems after various routes of exposure needs to be investigated using well-characterized NP.

With respect to this issue, scientists from the European Commission, Joint Research Centre co-authored a study published by the Journal of Nanoparticle Research, reporting on the generation of gold nanoparticles (Au-NP) aerosols for inhalation studies with the spark ignition technique. The Au-NP synthesis apparatus used was developed by the Comprehensive Pneumology Centre of the Institute for Lung Biology and Disease (Helmholtz Centre Munich).

According to the authors, the following aspects need to be addressed for inhaled NPs:

  • distribution and transport of NP within the respiratory tract,
  • their potential to cross the air–blood barrier (ABB) into the circulation and accumulate in secondary organs and tissues,
  • toxicological responses in the lungs and other tissues to the presence and/or accumulation of NP.

In order to study and understand these mechanisms, well-characterized aerosols of nanometer-sized particles are required, and in case of studies of quantitative biokinetics, a stably incorporated radiolabel is required.

In order to create radiolabelled Au-NP, gold electrodes suitable for utilisation in the spark ignition apparatus were irradiated with high energy protons at the MC40 Cyclotron of the JRC’s Institute for Health and Consumer Protection (Ispra). In this way 195Au radioisotopes were created in the electrode tips. This radioisotope has a half-life of 186 days, and thus is suitable for relatively long-term in vivo biokinetics studies. The MC40 cyclotron is only one of a few in Europe that has the characteristics necessary for such irradiations, since the vast majority of other cyclotrons cannot accelerate protons to the energies necessary for 195Au creation. During spark ignition, the 195Au radioisotopes are incorporated into the Au-NP in exactly the same was as non-active gold atoms, thus allowing synthesis of radiolabelled Au-NP with exactly the same physical and chemical characteristics as non-labelled particles.

Read the full article:

W. Möller, N. Gibson, M. Geiser, S. Pokhrel, A. Wenk, S. Takenaka, O. Schmid, A. Bulgheroni, F. Simonelli, J. Kozempel, U. Holzwarth, C. Wigge, S. Eigeldinger-Berthou, L. Mädler and W. G. Kreyling, Gold nanoparticle aerosols for rodent inhalation and translocation studies, Journal of Nanoparticle Research © Springer Science+Business Media, doi: 10.1007/s11051-013-1574-9

Photo: The MC40 Cyclotron Accelerator at JRC-IHCP, Ispra. Copyright EU 2010.

 
Document Actions
News: Highlights
New JRC report discusses labelling and reporting schemes for nanomaterials in consumer products in the EU
New JRC report discusses labelling and reporting schemes for nanomaterials in consumer products in the EU - May 19, 2014

JRC experts provide an overview on how EU legislation addresses nanomaterials, and recapitulate issues relevant for the on-going discussion on transparency and requests for more information regarding the use of nanomaterials in consumer products

Nanotechnology
JRC releases online web platform on nanomaterials
JRC releases online web platform on nanomaterials - Dec 19, 2013

The JRC released a first version of its web platform on nanomaterials which is mainly based on ...

Nanomaterials
JRC hosts international workshop on cyclotron research and nanoparticle radiolabelling
JRC hosts international workshop on cyclotron research and nanoparticle radiolabelling - Dec 17, 2013

Cyclotron research workshop “CYCLEUR” attracted a record number of participants

Nanoparticles
JRC hosts International Workshop on recent advances in Nanobiotechnology
JRC hosts International Workshop on recent advances in Nanobiotechnology - Dec 13, 2013

Current techniques and future developments discussed

Nanotechnology
Quantitative determination of the biodistribution of nanoparticles: could radiolabelling be the answer?
Quantitative determination of the biodistribution of nanoparticles: could radiolabelling be the answer? - Nov 08, 2013

In a recent article co-authored by CIC biomaGUNE (San Sebastian, Spain) and JRC, scientists from ...

Nanotechnology
see all
News: Publications
Updated list of materials in the JRC Nanomaterials Repository - Oct 27, 2011
The JRC MC40 Cyclotron and nanoparticle activation - Jul 04, 2011
Poster: Support to policy - Nanotechnology - Part 2 - Jun 16, 2011
Poster: Support to policy - Nanotechnology - Part 1 - Jun 16, 2011
E. Anklam: "Bridging Science and Policy to Support Innovation and Consumers’ Acceptance of Nanotechnology" - Jun 16, 2011
Updated list of materials in the JRC Nanomaterials Repository - May 26, 2011
see all
News: Multimedia
Watch our videoclip "Small materials, big impact" - Oct 17, 2011
see all
Deliverables: Reports
JRC Technical Report and Presentations: Symposium 'Safety issues and regulatory challenges of nanomaterials', May 2012 - Feb 02, 2013 - Jan 02, 2013
JRC report " Requirements on measurements for the implementation of the European Commission definition of the term 'nanomaterial'" - Sep 17, 2012
Joint JRC-EASAC Report "Impact of engineered nanomaterials on health: considerations for benefit-risk assessment" - Oct 18, 2011
Summary Report - Joint JRC Nano Event and 2nd ENPRA Stakeholders Workshop 2011 - Jan 10, 2011
Report of Workshop "Early Harvest of Research Results on Nanosafety" - Sep 13, 2010
see all
Watch our video "Small materials, big impact"
 

The video, issued in October 2011, illustrates JRC activities in support to safety assessment of nanomaterials in consumer products.

Watch it on You Tube, JRC channel